Paper Chromatography
Define paper chromatography
Paper chromatography is the separation of mixtures that contain multiple soluble substances.
What is the method for paper chromatography practical.
1. Draw a pencil line one centimeter from the bottom of the chromatography paper. This is the baseline.
2. Add a drop of the victim's urine to the line.
3. Place the chromatography paper in a beaker of water just below the baseline.
4. Leave to let the soluble substances in urine move up the paper.
5.Measure the distance between the baseline and the solent front and the distance between the baseline and the unknown substance.
6. Fill in the formula
7. Compare the RF value to the database of existing RF values.
Does the chromatography paper need to be above or below the baseline?
The chromatography paper needs to be below the baseline.
What makes the rate at which the solute move higher?
The rate at which the solute moves up the chromatography paper depends on soluability. The higher the soluability the higher the rate if which it moves.
What is the formula for Retention Value(RF)?
distance travelled by substance
÷
distance travelled by solvent
What should be used to draw the baseline and why?
A pencil should be used to draw the baseline so it doesn't dissolve in the water and move up the paper with the sample.
What shouldn't let happen to the water level?
You shouldnt let the water level reach above the baseline when dipped.