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pharmaceutical microbiology week 5

which media can a microbe grow in

liquid or solid

what slolid and liquid media called

solid media is called agar
liquid media is called broth

how can the organism grown be termined

by the content of the media and the conditions of incubation

what is culture media

Mixture of nutrients in solution
Contain nutrient used by microbes when grown in laboratory.

Contain buffer to maintain PH value.

Liquid (broth) or semi-solid (agar).

what is viable count

it is a common way assessing growth of single celled organism such as bacteria

why plating method can be inaccurate

as cells may clump together

we can determine the number of bacteria by.......

by counting the number of colonies

why do we have to dilute samples

to ensure plates are countable

assemblage of a host and the many other species living in or around it, which together form a discrete ecological unit

a holobiont

what is the code for ribosomal RNA

the 16s rRNA gene

The 16s rRNA gene required for....

the initiation of protein synthesis and the stabilization of correct codon-anticodon pairing in the A site of the ribosome during mRNA translation

why the 16s rRNA gene used as a bar code for microbial identification

- Universal conserved
- Long enough to discriminate between species.

- Data bases available.

what is the difference between resident and transient body macrobiota?

resident: relatively fixed types
of microorganisms, regularly found in a given area

at a given age


transient: nonpathogenic or potentially pathogenic microorganisms that inhabit some area for a short period

what is Normal microbiota?

Range of microorganisms evolves throughout life in a dynamic but stable balance (balance can be disrupted).

microflora depends on...

health, age, diet, climatic conditions

what is the four dominant phyla

actibobacteria
bacteroidetes

firmicutes

proteobacteria

what are the locations of normal microbiota on andin the human body

eyes
skin

nose and throat

large intenstine

urinary tract

reproductive systems

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