pharmaceutical microbiology week 5
which media can a microbe grow in
liquid or solid
what slolid and liquid media called
solid media is called agar
liquid media is called broth
how can the organism grown be termined
by the content of the media and the conditions of incubation
what is culture media
Mixture of nutrients in solution
Contain nutrient used by microbes when grown in laboratory.
Contain buffer to maintain PH value.
Liquid (broth) or semi-solid (agar).
what is viable count
it is a common way assessing growth of single celled organism such as bacteria
why plating method can be inaccurate
as cells may clump together
we can determine the number of bacteria by.......
by counting the number of colonies
why do we have to dilute samples
to ensure plates are countable
assemblage of a host and the many other species living in or around it, which together form a discrete ecological unit
a holobiont
what is the code for ribosomal RNA
the 16s rRNA gene
The 16s rRNA gene required for....
the initiation of protein synthesis and the stabilization of correct codon-anticodon pairing in the A site of the ribosome during mRNA translation
why the 16s rRNA gene used as a bar code for microbial identification
- Universal conserved
- Long enough to discriminate between species.
- Data bases available.
what is the difference between resident and transient body macrobiota?
resident: relatively fixed types
of microorganisms, regularly found in a given area
at a given age
transient: nonpathogenic or potentially pathogenic microorganisms that inhabit some area for a short period
what is Normal microbiota?
Range of microorganisms evolves throughout life in a dynamic but stable balance (balance can be disrupted).
microflora depends on...
health, age, diet, climatic conditions
what is the four dominant phyla
actibobacteria
bacteroidetes
firmicutes
proteobacteria
what are the locations of normal microbiota on andin the human body
eyes
skin
nose and throat
large intenstine
urinary tract
reproductive systems