liquid or solid
solid media is called agar
liquid media is called broth
by the content of the media and the conditions of incubation
Mixture of nutrients in solution
Contain nutrient used by microbes when grown in laboratory.
Contain buffer to maintain PH value.
Liquid (broth) or semi-solid (agar).
it is a common way assessing growth of single celled organism such as bacteria
as cells may clump together
by counting the number of colonies
to ensure plates are countable
the 16s rRNA gene
the initiation of protein synthesis and the stabilization of correct codon-anticodon pairing in the A site of the ribosome during mRNA translation
- Universal conserved
- Long enough to discriminate between species.
- Data bases available.
resident: relatively fixed types
of microorganisms, regularly found in a given area
at a given age
transient: nonpathogenic or potentially pathogenic microorganisms that inhabit some area for a short period
Range of microorganisms evolves throughout life in a dynamic but stable balance (balance can be disrupted).
health, age, diet, climatic conditions
nose and throat