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AUBF-CH05 CHEM EXAM

Principle of Glucose

Double Sequential enzyme reaction

Positive Color of Glucose

Green to brown

Principle of Bilirubin

Diazo reaction

Positive Color of Bilirubin

Tan or pink to violet

Principle of Ketones

Sodium nitroprusside reaction

Positive Color of Ketones

Purple

Principle of Specific Gravity

pKa change of a polyelectrolyte

Positive Color of Specific Gravity

Blue to Yellow

Principle of Protein

Proteins (Sorrensen's) Error of indicators

Positive Color of Protein

Blue

Principle of pH

Double indicator system

Positive Color of pH

Orange to Blue

Principle of Blood

Pseudoperoxidase activity of hemoglobin

Positive color of Blood

Uniform green & blue

Principle of Urobilinogen

Ehrlich reaction

Positive Color of Urobilinogen

Red

Principle of Nitrite

Greiss reaction

Positive color of Nitrite

Uniform Pink

Principle of Leukocyte

Leukocyte esterase

Positive color of Leukocyte

Purple

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