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BIOCHEM

It is called _______________ or ________________ chemistry

biological and physiological chemistry

it is the study of the chemical and molecular bases of life.

Biochemistry

Why do we need to study biochemistry?

To give the precise and correct medical intervention

It teaches how the biol0gical molecules like carbohydrates,protien,lipids nucleic acid give rise to different chemical processes in the living cell wich in turn gives rise to the complexity of life

Biochemistry

who was the first german scientist to perforn the biochemistry in 1903?

carl neuberg

It is a serious in a day to day life. And if not taken care of , this may lead to life threatening complications affecting cardiac and central nervous system. There is also a chance that the patient may go into seizure and coma

Hypoglycemia

Hypo --> ?
Hyper--> ?

Gly--> ?

Emia--> ?

gets down, gets up, glucose, blood

So, a thoroughunderstanding of the biochemical processes taking place in the body will help the nurse to have a ?

Patient centered approach in the treatment

Generally, knowing the normal processes that occur within the body will help us to _________________________that can occur during disease conditions so that an effective medical treatment can be facilitated.

Identify alterations

Study of biochemistry is significantly important for nurses because it facilitates the nurses to: (give 5)

- Understand the biochemical basis of health and illness
- interpret the biochemical diagnostics test reports

- contributes towards health promotion with biochemical aspect

- carry out effective assessment and planning for nursing needs

- implement the health education based on biochemical rationalization.

- Understand the biochemical basis of health and illness
- interpret the biochemical diagnostics test reports

- contributes towards health promotion with biochemical aspect

- carry out effective assessment and planning for nursing needs

- implement the health education based on biochemical rationalization.

Significance of studying biochem

Biochemistry teaches about homeostasis and electrolyte balance and a nurse should understand the importance of it in the body

true

Why does all the organs in the body function?

To maintain homeostasis

Biochemistry teaches about the normal and abnormal metabolite levels, and this knowledge helps the nurse to ___________________________by maintaining the fluid and electrolytes.

take special care

It is the ability or tendency of a living organism, cell, or group to keep the conditions inside it the same, despite any changes in the conditions around it, or this state of internal balance.

Homeostasis

The various biochemical tests like kidney function test, lipid profile test, liver function test, will help the nurse to diagnose diseases and the test results will help in assesing the needs of the paient to provide an effective care.

true

It is a sakit wherein bumababa ang sodium and chloride ay nawawala and electrolytes are decreasing. In treating this, Oresol and Pedialight are used.

Diarrhea

BGT BLOOD TEST:
- This test has normal value, random, and anytime you can get blood/blood samples.

RBS / Random Blood sugar

BGT BLOOD TEST:
- NPO is rquired, 6-7 hours fasting, nothing should be ingested 12 midnight to 6 or 7 am.

FBS / Fasting blood test sugar

BGT BLOOD TEST:
- It is the test the is the most preffered by doctors, it measures average 2-3 months of blood sugar (glucose level)

- Most relied result

Hb1AC/ hemoglobin A1C

BGT BLOOD TEST:
- BUN ( Blood Urea Nitrogen) and Creatinine are mataas and probably 800+ yung tinaas nung creatinine, so it means or the test shows that? what is the test used? (2 answers)

Kidney diseas, Kidney Function test, KDT

BGT BLOOD TEST:
- SGOT ( Serum Glutamic Oxaloacetic Transaminase)

- SGPT ( Serum Glutamae Pyruvate Transaminase)

- Bilirubin

- If all of this is mataas, what is the possible diagnosis? what test was used? (2 answer)

may tama liver, patient can have hepatitis, LFT / Liver Function Test

BGT BLOOD TEST:
- HDL (High Density lipoprotein)

- LDL (Low Density Lipoprotein)

- TG (Triglycerides)

- Cholesterol

- If mataas lahat there is a risk of? What test is used?

Heart attack, LPT/Lipid Profile Test

What are the good and bad Cholesterols?

HDL LDL

Every drug has a biochemical action and this knowledge will help in ____________________________ an the right duration of treatment a patient requires

Appropriate doses

It teaches the relation between health, diet and disease. It focuses on how each nutrient functions metabolically and biochemically.

Nutritional Biochemistry

In a nutrition, biochemistry is important in nursing because it gives knowledge about normal chemical process within the living cells and any change in it helps in the diagnosis of disease, thereby helping the nurse to provide an effective care to the patient.

true

It is the basic structural functional and biological unit of life and all organisms are made up of it

Cell

A-->M-->C-->T-->O-->S-->O

Atom, molecule, cell, tissue, organ, system, organism

It is the latin word that means small room
who discovered cells in 1665?

Cellula, Robert hooke

Review only:
- Cells are building block of life, where all the chemical processes occur.

- Cells take up biological molecules convert them into energy or use them into energy or use them to carry out specialized functions

- It also contains the hereditary material which determine the genetic characteristics

cell

Who developed the cell theory?

Matthias Jacob Schleiden and Theodor Schwann

All organisms are composed of one or more cells, and that all cells come from pre-existing cells. The vital functions of an organism occur within the cells and that all cells contain hereditary information necessary for cell functions and for transmitting from one generation to the next.

Matthias jacob Schleiden and Theodor Schwann

Every living things are made up of cell.
- some organisms are made up of just one cell __________

- some organisms are made up of many cells ___________

Unicellular, Multicellular

The human body is built with about how many cells?

30 to 40 million cells

These are cells have a true nucleus and it has a nuclear membrane within which there is well deffined chromosomes. These are called ______________ . They may be single -celled or multicellular organisms.

Eukaryotic cells

•those cells whose
nucleus is not distinct and their DNA is

not organized into chromosomes.

• They lack most of the membrane bound

organelles.

Prokaryotic cells

What are prokaryotic called?

prokaryotes

Cell whose nucleus is not distinct and their DNA is not organized into chromosomec. They lack most of membrane bound organelles.

prokaryotic cells

A typical eukaryotic cell, As seen under light microscope has two
major parts (2)

- Cell membrane
- The cutoplasm and its organelles

Difference of protoplasm and cytoplasm

Protoplasm - kasama si nucleus all organelles and nucleus in cutoplasm with nucleus
Cutoplasm - All organelles but nucleus consist of that part of the cell ,where the organelles except the nucleus are suspended to .

PART OF THE CELL
•It is a thin, elastic, semi permeable

membrane of 7.5 to 10 nanometers

thickness.

• It is a living membrane made up of

phospholipid bilayer embedded with proteins.

• It is a flexible membrane and so it can fold in or out.

• It is made up of 55 % proteins, 25 % phospholipids, 13 % cholesterol, 4% lipids and 3% carbohydrates.

Cell Membrane

FUNCTION OF WHAT PART OF THE CELL?
• It protects the cell contents from the surrounding environmement.

• It provides a shape to the cell.

• It is semi permeable and allows transport of certain substances into and out of the cell.

• It helps in forming cell junctions.

Functions of cell membrane

PART OF THE CELL
• It is a clear gelatinous fluid that fills the cell and surrounds the organelle.

• It contains 90 % water, dissolved

substances, minerals, sugar, irons,

vitamins, amino acids, proteins and

enzymes.

Cytoplasm

PART OF THE CELL
• It is the seat for many pathways like glycolysis and HMP

(Hexose monophosphate) pathway.

• It contains well organized structures called organelles which vary in size from a few nanometers to many micrometers and they are

specialized to carry out one or more vital functions of the cell.

Cytoplasm

PART OF THE CELL
• These are tiny, sausage shaped

structures of diameter 0.5 to 1 micrometer.

• It is found both in plant and animal

cells.

• It has a double membrane envelope: an inner membrane and an outer membrane.

Mitochodrion

PART OF THE CELL
• It is called as “Power house of the cell” as it generates energy in the form of AdenosineTriphosphate (ATP) which is required by all the cells.

Mitochondrion

Mitochodrion:
The inner membrane is folded and pleated

(double fold) and it is called _________.

cristae

MITOCHONRION
• It provides large surface area for different biochemical processes as it contains many oxidative enzymes.

• Within this membrane are the proteins involved in electron transport chain, ATP synthase and transport proteins.

• It is impermeable to molecules and ions but allows the free passage of carbon dioxide, oxygen and water.

• The metabolites are transported across the membrane with the help of transport proteins.

inner membrane/cristae

MITOCHONRION
• It is a smooth phospholipid bilayer enveloping the mitochondria.

• It had enzymes like monoamine

oxidase and NADH reductase (NADHNicotinamide adenine dinucleotide + Hydrogen)

Outer membrane

MITOCHONRION
• An intrinsic protein called _________ form the channels that makes the membrane permeable to solutes and metabolites

• It allows the free passage of substances with molecular weight of less than 10,000.

• The membranes create two

compartments.

Porin

The space between the outer and inner membrane is called the?

intermembrane space

The inner cavity of mitochondria is called the?

matrix

PART OF THE CELL:
It is here that oxidative phosphorylation occus. (Releasing energy by oxidizing nutrients for ATP

synthesis)

Mitochodrion

PART OF MITOCHONDRIA:
• It is packed with many enzymes like pyruvate dehydrogenease, pyruvate

carboxylase, enzymes for oxidation of fatty acids, aminoacids and enzymes of

citric acid cycle.

• It also contains the mitochondrial genome, mitochondrial ribosomes,

tRNAs, dissolved oxygen, carbon dioxide and water.

Matrix

WHOSE FUNCTION IS THIS?
–It is the seat for the Kreb's cycle (citric acid cycle)

–It contains enzymes for Oxidative phosphorylation which helps in producing energy rich ATP molecules

–It provides intermediates for synthesis of cytochrome,

chlorophyll, hemoglobin and

steroids

–Amino acids like glutamate

are synthesized in it from alpha ketoglutarate and oxaloacetate

–Many fatty acids are synthesized in the matrix

MITOCHODRION

Can mitochondria store and release calcium? When is it released?

Yes, It is released when required

PART OF THE CELL
•It is a network of tubular and vascular structures extending from outer

membranes of nucleus to the cell membrane.

• It is seen to be spread throughout the cytoplasm and it provides a large surface area for various physiological activities.

Endoplasmic reticulum

The inside of the vesicles and tubules
is filled with an _______________ ____________

Endoplasmic matrix

PART OF CELL SPECIFICALLY ER
•When the ribosomes are attached on the outer surface of mebrane of the

endoplasmic reticulum, it is called as?

Rough Endoplasmic Reticulum

PART OF THE CELL SPECIFICALLY ER
•When there are no ribosomes attached to the endoplasmic reticulum, it is called as?

Smooth Endoplasmic Reticulum

FUNCTIONS OF WHAT? (PART OF ER)
–It helps in transporting proteins from ribosomes to Golgi bodies.

–Proteins that enters in here undergoes processing, folding and sorting

Rough Endoplasmic reticulum

WHOSE FUNCTION IS THIS? (PART OF ER)
–It is involved in the synthesis of lipids, including cholesterol and phospholipids

–In some cells, It helps in the synthesis of steroid hormones from cholesterol

–In the cells of liver, It helps in detoxifying drugs and harmful chemicals.

Smooth Endoplasmic reticulum

PART OF THE CELL
• It is a stack of membranous sac, like a pile of discs.

• It is present between endoplasmic reticulum and cell membrane.

• Like endoplasmic reticulum, it is a single membrane bound structure.

• In animal cells, it is present around the nucleus while in plant cell it is scattered throughout the cell.

• These cell organelles pack and sort the proteins before they are sent to their destinations.

Golgi Apparatus

WHOSE FUNCTION IS THIS?
(PART OF THE CELL)

–The proteins that enter it from RER is modified, processed, sorted and transported in the form of vesicles to the cell membrane and other destinations.

–Glycolipids, sphingomyelin are synthesised within it.

–In plant cells, it help in the synthesis of polysaccharides needed by the cell wall.

–It has a role in the synthesis of carbohydrates like galactose.

–Primary lysosomes develop from mature Golgi bodies.

–It plays an important role in lipid trafficking.

Golgi apparatus

PART OF THE CELL
• They are spherical shaped organelles seen either free in the cytoplasm or attached to RER.

• They are found in eukaryotes and prokaryotes.

• They are synthesised by the nucleolus.

• They link the amino acids together in the order that is specified by

the messengers RNA.

• They are made up of two subunits - a small sub unit and a large sub unit.

(CLUE: RELEASED BY THE NUCLEUS)

Ribosomes

COMPLETE IT
(IT ABOUT RIBOSOMES)

- The small sub unit reads the ________ while the large subunit ____________ the

amino acids to form large ______________.

- The ribosomal sub units are made up of one or more ________ and proteins.

• The small sub unit reads the mRNA while the large subunit assembles the
amino acids to form large polypeptide.

• The ribosomal sub units are made up of one or more eRNA and proteins.

PART OF THE CELL
It is the site for protein synthesis.

Ribosomes

PART OF THE CELL
• They are tiny sac like organelles of size 0.5 to 1.5 µm, which are membrane bound and found in hundreds in a single cell.

• They are formed from the Golgi bodies as small vesicles which bud off from them.

• Within the sac there are several hydrolytic enzymes that breakdown macromolecules like nucleic acid, proteins and polysaccharides.

Lysosomes

PART OF THE CELL
• They are also called as “suicidal bags” as enzymes contained in them can digest the cell's own material when damaged or dead.

• The important enzymes in it are DNAase, RNA-ase, protease, lipase, glycosidase, phosphatase, sulfatase which are synthesized in the

endoplasmic reticulum and then transported to the Golgi bodies.

Lysosomes

WHOSE FUNCTION IS THIS? (PART OF THE CELL)
–It helps in the digestion of food releasing enzymes.

–They digest worn out organelles

–It helps in the defense, by digesting germs

–It helps sperm cells in entering the egg by

breaking through the egg membrane

–It provides energy during cell starvation by the digestion of its own cell parts.

Lysosomes

PART OF THE CELL
• It is a type of microbody which is small, spherical shaped, with single membrane and of size 0.5-1.5 µm.

• They are found in both animal and plant cells.

• Just like lysosomes they contain many enzymes which help in biological reactions.

• They are formed from the endoplasmic reticulum unlike lysosomes which are formed from the Golgi bodies.

Peroxisome

WHOSE FUNCTION IS THIS?
(PART OF THE CELL)

–The enzymes found in ____________ are usually used for different metabolic reactions and for digesting different materials in the

cell.

–They help in the oxidation of many substances resulting in the formation of hydrogen peroxide as a by product.

–But, it contains enzyme peroxidase or catalase which decomposes this harmful hydrogen peroxide into water and oxygen or uses it to oxidize other organic compounds like phenol, alcohol, formaldehyde, etc.

Peroxisome

WHOSE FUNCTION IS THIS?
(PART OF THE CELL)

–They are also involved in the catabolism of fatty acids (beta oxidation), D-aminoacids and polyamines.

–They are needed in the synthesis of plasmalogens (type of ether phospholipid

needed for functioning of brain and lungs.)

–They participate in the synthesis of cholesterol, bile acids and myelin.

–In plants, it helps in the photorespiration and symbiotic nitrogen fixation.

Peroxisome

PART OF THE CELL (CYTOSKELETON)
• They are long, hollow cylinders of size 24 nm in diameter and can grow up to a length of 50 µm.

• They are found in eukaryotic cells.

Microtubules

PART OF THE CELL (CYTOSKELETON)
• They are made up of two globular proteins namely

α-tubulin and β-tubulin.

• Along the _________ axis

tubulins are joined end to end to form protofilaments.

Microfilaments

PART OF THE CELL?
•It is a structure that helps cells maintain their shape and internal organization, and it also provides mechanical support that enables cells to carry out essential functions like

division and movement.

• They are organized by

microtubule organizing

structures, primarily the

centrioles.

Cytoskeleton

WHOSE FUNCTIONS ARE THESE? (PART OF THE CELL?)
–It provides mechanical support to the cell

–It helps in the organization of cytoplasm

–They help in the segregation of chromosomes during mitosis

–They are used for locomotion (movement from one place to another) when present)

Cytoskeleton

PART OF CYTOSKELETON
•These are small rod like structures of size 4-7nm in diameter found in the cytoplasm of all eukaryotic cells, forming a part of the cytoskeleton.

• They are made up of protein, Actin (contractile protein)

Microfilaments

PART OF CYTOSKELETON
–It provides support and shape to cell

–Along with myosin, it helps in contraction

–It helps in cytokinesis (a physical process of cell division)

Microfilaments

PART OF CYTOSKELETON
•They are part of the cytoskeleton in the cytoplasm, also surrounding the nucleus and extending to the cell membrane.

• They are made up of different types of fibrous proteins unlike microtubules

which are made up of actin.

• They are of size 8-12 nm in diameter.

• They are found in hair, nails, scales and skin since they have high tensile strength.

Microfilament

PART OF CYTOSKELETON
• Due to their rope like structure they provide mechanical strength to the cells and help cells to withstand stress like stretching and changing shape.

• e.g. Keratin filaments in skin and epithelial cells.

Microfilaments

PART OF THE CELL
• It is cylindrical in shape and of length 0.5 micrometer.

• it is present in all animal cells just outside the nucleus.

• It does not have a membrane.

• All centrioles are made up of protein strands called tubulin.

• Each of it has 9 sets of inter connected peripheral tubules and each set has 3 micro tubules arranged at

definite angles making the shape of a W2 cylinder.

Cetrioles

PART OF THE CELL
•It has its own DNA and RNA and therefore, self duplicating. ____________

• Two centrioles when oriented at a right angle, its is called ____________

centrioles, centrosome

WHOSE FUNCTION IS THIS? ( PART OF THE CELL)
▪It is involved in cell division.They are seen in the process of both meiosis and mitosis.

▪ It helps in the formation of cilia and flagella.

▪It helps in the organization and alignment of microtubules within the cell.

Centrioles

PART OF THE CELL
• It is a membrane enclosed fluid filled sac present in animal and plant cells including fungi.

• It contains organic and inorganic molecules within it.

• They do not have particular size or shape but adjust themselves according to the need of the cell.

Vacuole

WHOSE FUNCTION IS THIS? (PART OF THE CELL)
▪It helps in removing waste products from the cell

▪It isolates substances that are harmful to the cell

▪It holds water and waste products within it

▪It helps in maintaining the internal pH of cell

▪It helps to maintain hydrostatic pressure within the cell

▪It plays a major role in autophagy by maintaining a balance between biogenesis and degradation.

Vacuoles

PART OF THE CELL
- It is a specialized double membranebound protoplasmic body present at the center of the cell.

- It is known as the cell's information center as it houses the chromosomes.

nucleus

PART OF THE NUCLEUS
- The double membrane around it is called __________ ___________or __________ _____________.

- It is made of proteins and lipids,

- It enclose the nucleus to keep it separate from surrounding materials off the cell.

Nuclear membrane or Nuclear Envelop

PART OF THE NUCLEUS (NUCLEAR MEMBRANE)
- The ___________________ is continuous with endoplasmic reticulum and it has ribosomes attached on the outer surface.

- It has several large nuclear pores through which nuclear transport of large molecules, small molecules and ions occur.

Outer Membrane

The space between the nuclear membranes is called ____________ _________ and it is continuous with the lumen of rough endoplamic reticulum.

Perinuclear Space

Within the nuclear membrane is a jelly like substance called ________________ or nucleoplasm.
+ Within it, there is a network of chromatin fibrils which condense to form chromosomes during cell division

karyolymph

PART OF THE NUCLEUS/PART OF THE CELL
It is present within the nucleus.

• It does not have a membrane around it.

• It synthesizes rRNA and assembles it.

• It regulates the synthetic activity of nucleus.

Nucleolus

WHOSE FUNCTION IS THIS? (PART OF THE CELL)
- It controls the hereditary charateristics of an organism

-Protein synthesis, cell division, growth and differentiation occurs in it

- Stores heredity materials in the form of DNA

- t is the site for transcription in protein synthesis

- Nucleolus helps in the synthesis ofribosomes

- It regulates the integrity of genes and gene expression

Nucleus

It is a single-celled organism that does not have a 'true nucleus'.
e.g. Bacteria, Archaea.

- It does not contain any membrane bound organelles like mitochndria, nucleus, endoplasmic reticulum etc.

- They have many ribosomes scattered throughout their cytoplasm and nucleoid which contains the DNA.

Prokaryote

What are the 9 parts of prokaryotic cell?

Flagellum pili cell membrane capsule cellwall cytoplasm ribosme nucleoid plasmid

PART OF THE PROKARYOTIC CELL
It is long whip like structure that helps in locomotion

Flagellum

PART OF THE PROKARYOTIC CELL
Small hair like structure present on the surface which helps in attaching to the surface of other bacteria.

pili

PART OF PROKARYOTIC CELL
It surrounds the cytoplasm and regulates the flow of substance in andout of the cell.

Cell membrane

PART OF THE PROKARYOTIC CELL
- It is a polysaccharide layer that is outside the cell envelope.

- It enhances the ability of bacteria to cause disease

Capsule

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