Sysmex 9100
what does the sysmex use to work
hydrodynamic focusing flow cytometry, and SLS hemoglobin methods
HCT formula
RBC x MCV /10
MCH formula
(HGB/RBC) x10
MCHC formula
(HGB/HCT) x100
MCV formula
(HCT/ RBC) x10
what is the formula used to correct the wbc count if >5 NRBCs (or Megakaryocytes) are present
False WBC# x 100/ NRBC +100
++++
outside of reportable range
----
analysis error
@
parameters exceeded the limit of the instruments linearity
+/-
result critically high/ low
*
low reliability of data/ invalid results
&
WBC: signifies counting took longer than average| PLT: signifies fluorescent dye was used
Blank
presence of clot/ short or improper sample
IG present
immature granulocytes present
MCHC > 37.5%
may imply hereditary spherocytosis or a cold agglutinin
Retic# reference intervals
0.5-2.5%
XN-20 (L) has ___________________-, which XN-10 (R) doesnt
Fluorocell WPC
cellpack DCL
Diluent; rinsing agent
cellpack DFL
Diluent; used in combination with either Fluorocell RET or PLT
Sulfolyser (SLS)
Determination of HGB concentration
Lysercell WNR
used with fluorocell WNR to hemolyze rbcs and stain WBCS, basophils, and NRBCs
Lysercell WDF
used with fluorocell WDF; hemolyzes RBCs and dyes wbc contents
Fluorocell WNR
stains all nucleated cells
Fluorocell WDF
stains leukocytes
Fluorocell RET
stains reticulocytes
Fluorocell PLT
stains platlets
Fluorocell WPC
stains wbcs in order to distinguish atypical lymphs and blast cells