Bacteriology Laboratory: Module 6: Method of Sterilization
renders an item or material safe to handle
decontamination
the level of microbial contamination is reduced enough
decontamination
temperature which in a given time destroys all the bacteria present
thermal death point
minimum time requird to fill a suspension of organism of a given temperature in a specified environment
thermal death time
process whereby all forms of microbial life including bacterial spores are killed
sterilization
free of life kind
sterile
agents that kill bacterial
bactericidal agents
agents that inhibit bacteria growth and multiplication
bacteriostatic agents
active form
vegetative cell
normal growing cell that is functional
vegetative cell
inactive form
spores
dormants and produced under unfavorable environmental conditions
spores
presence of pathogenic microbes in living tissue
septic
absence of pathogenic microbes in living tissue
aseptic
non living things
inanimate objects
also known as germicide
disinfection
probability of a microorganism surviving on an item ubjected to treatment in less than
sterility assurance level
What are the physical methods of sterlizatiom
handwashing incineration dry heat autoclaving fractional filtration ionizing sedimentatiom moist heat
hand soap + water
handwashing
for visibly soiled hands
handwashing
kills microbial life by oxidation
dry heat
temperature of dry heat for how many hours
160-180 C for 1-2 hours